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Image Search Results
Journal: Scientific Reports
Article Title: Targeted mutagenesis in soybean using the CRISPR-Cas9 system
doi: 10.1038/srep10342
Figure Lengend Snippet: Construction of binary vectors for genome editing in soybean. Cas9 fused with a single nuclear localization signal (NLS) is expressed with a Cauliflower mosaic virus 35s (CaMV 35s) promoter. Synthetic guide RNA (sgRNA) is derived using U6 promoters. ( a ) Arabidopsis thaliana U6-26 promoter ( b ) Glycine max U6-10 promoter. Sequences containing two Bsa I sites are located between the U6 promoter and the sgRNA scaffold. These sequences can be easily replaced with a gene-specific sgRNA seed. LB: left border; RB: right border.
Article Snippet: Arabidopsis U6-26 and soybean U6-10 promoters with
Techniques: Virus, Derivative Assay